【 KCI 】Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs > 학술지 논문

【 TITLE 】Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs

【 JOURNAL 】Journal of Veterinary Nursing

​​【 NAME 】Seul-Gi Yang, Jae-Hun Choi, Young-Seo Jo, Ye-Won Kim, Dong-Mok Lee, Hyo-Jin Park, and Deog-Bon Koo

【 PUBLISHED 】 December 2022​

【 ISSN 】2950-9033

【 DOI 】10.56878/jvn.2022.1.2.51

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​【 ABSTRACT 】​

 Mitochondria plays an important role in regulating cytoplasmic calcium (Ca2+) homeostasis for ATP production during early embryonic development in mammals. Accumulation of mitochondrial Ca2+ induces mitochondrial dysfunctions. However, its relevance with change of mitochondrial Ca2+ in matured and fertilized oocytes has not been reported. The aim of this study was to identify the changes of mitochondrial Ca2+ level by using Rhod-2 staining analysis which is known as mitochondrial Ca2+ indicator in matured and fertilized oocytes in vitro. Here, we divided into two groups (Group 1: G1, compact cumulus cells and homogeneous cytoplasm and Group 2: G2, non-compact cumulus cells and heterogeneous cytoplasm) according to the morphological difference of COCs. Meiotic maturation rate decreased in G2 compared to G1 (p<0.001). Average number of Rhod-2 spots in matured and fertilized oocytes was higher in G2 than in G1 (6 h after IVF: p<0.05). We also investigated the detailed expression pattern of Rhod-2 spots dividing it into four parts (n<10, 10≤n< 20, 20≤n<30, and 30≤n). The ratio of fertilized oocytes with less than 10 Rhod-2 spots was higher in G1 (p<0.05). In addition, the Rhod-2 intensity in matured and fertilized oocytes in G2 were all higher than that in G1 (matured oocytes; p<0.001 and zygotes; p<0.05). Based on these results, we confirmed that morphological difference of immature oocytes was correlated with mitochondrial Ca2+ accumulation during porcine oocyte maturation and fertilization in vitro.

​【 Keywords 】Antioxidant enzyme, Cumulus cell expansion, Oocyte maturation, Ovarian extract, Pig