국외논문 【 SCIE 】 Native Plants (P. amurense and H. japonicus) Extracts Act as …
페이지 정보
작성자 최고관리자 작성일 19-07-15 13:54 조회 405회 댓글 0건본문
【 TITLE 】Native Plants (P. amurense and H. japonicus) Extracts Act as Antioxidants to Support Developmental Competence of Bovine Blastocysts
【 JOURNAL 】Asian-Australasian journal of animal sciences (AJAS)
【 NAME 】Geon-Yeop Do, Jin-Woo Kim, Hyo-Jin Park, Seung-Bin Yoon, Jae-Young Park, Seul-Gi Yang, Bae Dong Jung, Yong-Soo Kwon, Man-Jong Kang, Bong-Seok Song, Sun-Uk Kim, Kyu-Tae Chang and Deog-Bon Koo
【 PUBLISHED 】01 Sep 2017
【 ISSN 】1976-5517
【 DOI 】https://doi.org/10.5713/ajas.16.0985
【 ABSTRACT 】
Objective
Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extracts on developmental competence and quality of preimplantation bovine embryos.
Methods
After in vitro fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with P. amurense (0.01 μg/mL) and H. japonicus (0.01 μg/mL). The effect of this supplementation during in vitro culture on development competence and antioxidant was investigated.
Results
We observed that the blastocysts rate was significantly increased (p<0.05) in P. amurense (28.9%±2.9%), H. japonicus (30.9%±1.5%), and a mixture of P. amurense and H. japonicus (34.8%± 2.1%) treated groups compared with the control group (25.4%±1.6%). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both P. amurenseand H. japonicus extract treated embryos.
Conclusion
These results suggest that proper treatment with P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.
【 KEYWORDS 】 Bovine Embryo, Phellodendron amurense, Humulus japonicas, Reactive Oxygen Species (ROS), Antioxidants
- 이전글【 SCI 】 Melatonin improves the meiotic maturation of porcine oocytes by reducing endoplasmic reticulum stress during in vitro maturation
- 다음글【 SCI 】 Ganglioside GM3 Induces Cumulus Cell Apoptosis through Inhibition of Epidermal Growth Factor Receptor-mediated PI3K/AKT Signaling Pathways during in vitro Maturation of Pig Oocytes
댓글목록
등록된 댓글이 없습니다.