【 SCI 】 Ganglioside GM3 Induces Cumulus Cell Apoptosis through Inhibition of Epidermal Growth Factor Receptor-mediated PI3K/AKT Signaling Pathways during in vitro Maturation of Pig Oocytes > 학술지 논문

【 TITLE 】Ganglioside GM3 Induces Cumulus Cell Apoptosis through Inhibition of Epidermal Growth Factor Receptor-mediated PI3K/AKT Signaling Pathways during in vitro Maturation of Pig Oocytes

【 JOURNAL 】Molecular Reproduction and Development

【 NAME 】Hyo‐Jin Park, Sung‐Kyu Chae, Jin-Woo Kim, Seul-Gi Yang, Jae-Min Jung, Min-Ji Kim, Gabbine Wee, Dong-Seok Lee, Sun-Uk Kim and Deog-Bon Koo

【 PUBLISHED 】01 Aug 2018​

【 ISSN 】1040-452X

【 DOI 】10.1002/mrd.22848

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​【 ABSTRACT 】

​ Gangliosides are components of the mammalian plasma membrane that help regulate receptor signaling. Ganglioside GM3, for example, plays an important role in initiating apoptosis in cancer cells; however, physiological roles for GM3 in normal processes, such as during pig oocyte maturation, are not clear. The aim of this study was to investigate the functional link between GM3 and cellular apoptosis in porcine cumulus-oocyte-complexes (COCs) during in vitro maturation. Our results indicated that denuded oocytes possess less ST3GAL5, a GM3-synthesizing enzyme, than cumulus cells or COCs after 44 hr of in vitro maturation. GM3 also affected the meiotic maturation of cultured pig oocytes, as evaluated by orcein staining. In vitro treatment of COCs with exogenous GM3 also reduced cumulus cell expansion, the proportion of meiotic maturation, and increased cumulus cell transcription of PTX3, TNFAIP6, and HAS2. Interestingly, GM3 treatment reduced the expression of Epidermal growth factor receptor (EGFR)-mediated Phosphoinositide 3-kinase/AKT signaling proteins in COCs in a concentration-dependent manner, instead increasing the abundance of pro-apoptotic factors such as AIF, activated Caspase 9, cleaved PARP1, and Caspase 3 were. Thus, GM3 might affect porcine oocyte maturation via suppression of EGFR-mediated PI3K/AKT signaling and/or induction of apoptosis during in vitro maturation.

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​【 KEYWORDS 】PI3K/AKT, Pig, apoptosis, ganglioside GM3, in vitro maturation​