2023년 한국동물생명공학회 학술 대회 [Jae-Hun Choi] > 학회발표

Paclitaxel supports the delayed bovine embryonic development impaired growth until blastocyst stage through F-actin derived cytoskeleton stabilization after in vitro fertilization 

Jae-Hun Choi^1,2, Hyo-JinPark^1,2, Seul-Gi Yang^1,2, Ye-Won Kim^1,2, Dae-Wook Kim^1,2, Deog-Bon Koo^1,2,3 

¹ Department of Biotechnology, College of Engineering, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, Republic of Korea, ² Institute of Infertility, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, Republic of Korea, ³ Department of Companion Animal Industry, College of Natural and Life Sciences, Daegu University, Gyeongbuk 38453, Republic of Korea

Our previous study showed that the structure of F-actin derived cytoskeleton plays a central role in porcine embryonic developmental competence for blastocyst formation aspects of cell biology. While the intricate process of cleaved embryos after in vitro fertilization (IVF) requires a properly regulated changes in F-actin derived cytoskeleton, little is known about the contributions of different F-actin stabilization using paclitaxel during embryogenesis. Preferentially, at 48 h of culture after in vitro fertilized bovine embryos, they were divided into two groups based on the number of cleaved blastomeres (Group A : less than 8 cells and Group B : over than 8 cells). As expected, we found that bovine embryos over than 8 cells stage at 48 h after IVF were viable until blastocyst stage (Group B: 43.6%, n=105/241). But most remained the cleavage-arrested blastomeres due to delayed bovine embryos development less than 8 cell stage within 48 h after IVF (Group A: 0.94%, n=8/218). To detect the cytoskeleton activity, we measured the F-actin and vimentin protein expressions using immunofluorescence staining in cleavage and blastocyst stages. Both F-actin and vimentin were significantly elevated in Group B compared with Group A in cleavage and blastocyst stages. Based on these results, we investigated whether paclitaxel could ameliorate the developmental delay of extracorporeal bovine embryos. The 10 nM paclitaxel exposure significantly improved the blastocyst development rate from Group A (p<0.05; Non-treated: 0.9 ± 1.1% vs paclitaxel-treated: 3.2 ± 2.0%, 3.5-fold) with accompanied by F-actin stabilization at adhesion-junction site in cleaved embryos. Unfortunately, we found no significant overall differences for effect of paclitaxel on embryonic developmental ability in Group B. Therefore, these findings suggest that 10 nM paclitaxel exposure during IVC improves the developmental competence of delayed bovine cleavage embryos through F-actin stabilization.

*This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF-2021R1C1C2009469, NRF-2022R1A2C1002800, and NRF-2021R1A6A3A01087623) funded by and the Ministry of Science and ICT, Republic of Korea.

Key words: Paclitaxel, F-actin, Cytoskeleton, Bovine embryo