2022년 한국동물생명공학회 학술 대회 [Ji-Hyun Shin] > 학회발표

Differential expression patterns of cytoskeleton-related genes for meiotic maturation in porcine poor quality and aged oocytes in vitro

Ji-Hyun Shin1,2, Hyo-Jin Park1,2, Seul-Gi Yang1,2, Jae-Hun Choi1,2, Young-Seo Jo1,2, Ye-Won Kim1,2, and Deog-Bon Koo1,2,*

1Department of Biotechnology, College of Engineering, Daegu University,

201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, Republic of Korea

2Institute of Infertility, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, Republic of Korea

   During mammalian oocyte maturation, the cytoskeleton plays an important role in spindle assembly. Aduucin α (ADD1) is known to be involved in mitotic spindle assembly as one of the F-actin binding proteins. While many studies examined the arrangement of spindle related to microtubule in oocyte maturation, the loss of F-actin cytoskeleton organization in porcine postovulatory aged oocytes with deteriorated quality during meiosis has not been reported. First, porcine oocytes were divided into two groups (good quality: G1 vs poor quality: G2) based on with/without cumulus cells. The G2 oocytes group caused defects in meiotic maturation capacity (p < 0.05, G1: 82.7 ± 6.3% vs G2: 64.6 ± 5.5%) and impaired (p < 0.05) cytoskeleton organization including spindle assembly and actin dynamics compared to G1 oocytes group. Next, we used aged oocytes cultured for an additional 24 h after in vitro maturation to confirm the changes in cytoskeleton organization in the aged model. Likewise, in aged oocytes, failure of spindle assembly was increased and the expression of F-actin and ADD1 was significantly decreased. In addition, we analyzed specific expression patterns of aging and cytoskeletal-associated genes such as CLU, FAM111A, MAP7, and SKA1 using RNA sequencing analysis. These results indicated that F-actin and actin-binding protein ADD1 play a key role in meiotic maturation via microtubules related to spindle assembly in poor-quality and aged oocytes. Moreover, we suggested diverse aging and cytoskeleton-related genes in porcine oocytes can be effective factors to improve the quality and subsequent developmental capacity by preventing postovulatory aging.

*This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF-2021R1C1C2009469, NRF-2022R1A2C1002800, and NRF-2021R1A6A3A01087623) funded by and the Ministry of Science and ICT, Republic of Korea.

Key words) F-actin, microtubule, spindle assembly, oocyte aging, in vitro maturation, RNA sequencing

E-mail : jihyn098@naver.com (Ji-Hyun, Shin).